INTRODUCTION:

Terminalia arjuna is a tree of the genus Terminalia. It is commonly known as Arjuna [1] or arjuna tree. The arjuna grows to about 20–25metres tall; usually has a buttressed trunk, and forms a wide canopy at the crown, from which branches drop downwards. It has oblong, conical leaves which are green on the top and brown below; smooth, grey bark; it has pale yellow flowers which appear between March and June; its glabrous, 2.5 to 5cm fibrous woody fruit, divided into five wings, appears between September and November.^1,2The arjuna was introduced into siddha by saint Agastiyar through his prose Gunavakatam and in Ayurveda as a treatment for heart disease by Vagbhata (c. 7th century CE).³ It is traditionally prepared as a milk decoction.⁴

In the Ashtānga Hridayam, but was also mentioned in many ancient Hindu vedas, and was a known practice for thousands of years, passed down by tradition, before vagbhata mentioned it in his writings. Vagbhata mentions arjuna in the treatment of wounds, hemorrhages and ulcers, applied topically as a powder. The plant contains various Phytoconstituent like tannins, cardenolide, triterpenoid saponins (arjunic acid, arjunolic acid, arjungenin, arjun glycosides), flavonoids (arjunone, arjunolone, luteolin), gallic acid, ellagic acid, oligomeric proanthocyanidins (OPCs), phytosterols, calcium, magnesium, zinc, and copper.

With increasing demand for herbal products in medicines and cosmetics there is an urgent need for standardization. So the aim of the work is to develop a simple, rapid, selective and cost effective HPTLC method for the quality evaluation of herbal products containing Andrographolide in tablet formulation.

MATERIAL AND METHOD:

Material:

Himalaya Arjuna tablet was procured from local market of Nashik, MS, India. Chemicals like Methanol, Sodium sulphate were purchased from local market of Nashik.

HPTLC:

CAMAG Linomat 5 “Linomat 5-210175” S/N 210175 (1.00.13) sytem was use for the study purpose.

Sample and standard preparation:

Standard preparation:

Weigh exactly 75mg of Arjuna extract. Add 5ml of Methanol and sonicate for 10 min. Filter through anhydrous Sodium Sulphate. Collect the filtrate in 10ml volumetric flask. Wash the filtrate with 2ml of Methanol and collect filtrate. Make up the volume upto 10ml mark and use for TLC Spotting

Sample preparation:

Weigh accurately tablet powdered previously equivalent to 250mg Arjun extract (570mg as per average wt of tablet). Add 5ml of Methanol and sonicate for 10 min. Filter through anhydrous Sodium Sulphate. Collect the filtrate in 10ml volumetric flask. Wash the filtrate with 2 ml of Methanol and collect filtrate. Make up the volume upto 10ml mark and use for TLC Spotting. Scanned at 254nm.

Chromatography:

TLC Aluminum pre coated plate with Silica gel 60 GF254 (20x10 cm2; 0.2mm thick) was used with Toluene: Methanol (4:3) v/v as a mobile phase. Methanolic extract of sample and Andrographolide was applied on TLC plate using Linomat V applicator.

Fig. No.01: Himalaya Arjuna Tablet

Fig. No. 02: Termenelia Arjuna Methanolic extract

Fig. No. 3: Auto generated scan of Himalaya Arjuna tablet and Arjuna extract

RESULTS AND DISCUSSION:

Table no. 01: As per Chromatogram RF Selected as per Reference of ICMR.

Track No,

Wavelength

(nm)

Rf value

Conc.

Peak area (AU)

Color band

254

0.29

7.5 mg.ml

270.2

Green

254

0.30

57 mg/ml

903.8

Green

Calculations: (At 254nm)

% of Arjuna extract= Area of sample X Conc. of STD X 100/Area of STD X Conc. of Sample

= 903.8 X 7.5 X 100/270.2 X 57

= 44.01% w/w

Avg wt of Tablet: 570mg as per label claim Each Tablet Content 250mg Arjuna Extract.

By Considering 570mg Tablet =100%

X mg Arjuna Extract = 44.01%

By cross multiplication: 44.01 x 570/100= 250.85mg

Arjuna Extract:

The various mobile phases tried for the Arjuna tablet toluene: methanol (4:3) v/v shows green color band at an Rf value of 0.29 very efficiently from the tablet formulation as well as Methanolic extract of Arjuna. Sharp peaks of Andrographolide were obtained when TLC plate was scanned at 254nm. Quantity of Andrographolide from tablet formulation and alcoholic extract was obtained automatically and was used in formula to find out percentage purity of Andrographolide from Arjuna tablet and Arjuna extract.

CONCLUSION:

The proposed HPTLC method is simple, rapid, accurate, reproducible, selective and economic and can be used for routine quality control analysis of Andrographolide from marketed preparations.

ACKNOWLEDGEMENT:

Author wish to express their sincere thanks to Mr. Sampat Shinde of Reve Pharma, Musalgaon, Sinnar for providing necessary facilities to carry out research work.

REFERENCES:

1. Biswas, Moulisha; Biswas, Kaushik; Karan, Tarun K; Bhattacharya, Sanjib; Ghosh, Ashoke K; Haldar, Pallab K (2011). "Evaluation of analgesic and anti-inflammatory activities of Terminalia arjuna leaf". Journal of Phytology. 3(1): 33–8.

2. M.P. Shiva, NWFP, and EC-FAO Partnership Programme. "Non-wood forest products in 15 countries of Tropical Asia". Food and Agriculture Organization of the United Nations.

3. Jump up to: "Arjuna". Todd Caldecott. Archived from the originalon 2012-09-14. Retrieved 2012-11-26.

4. Kapoor LD. Handbook of Ayurvedic Medicinal Plants. Boca Raton, FL. CRC Press; 1990:319-320.

5. Bone K. Clinical Applications of Ayurvedic and Chinese Herbs. Warwick, Queensland, Australia. Phyto-therapy Press; 1996:131-133.

Received on 26.10.2020 Revised on 25.11.2020

Asian Journal of Pharmaceutical Analysis. 2021; 11(2):156-158.

DOI: 10.52711/2231-5675.2021.00027